Neurturin基因的克隆及其在Vero细胞中的表达Cloning of Neurturin Gene and Its Expression in Vero Cells
马开利;孙茂盛;施锐;张莹;杨芳;奎翔;谭振国;李鸿钧;
摘要(Abstract)：
目的克隆Neurturin(NTN)基因,并在Vero细胞中表达。方法用RT-PCR方法扩增hNTN cDNA,并克隆至pcDNA3真核表达载体中,经Lipofectamine2000转染Vero细胞,挑选稳定表达克隆,用RT-PCR及免疫荧光分析鉴定,并对转染后细胞的形态及生长状况进行分析。结果重组质粒pcDNA3/hNTN转染Vero细胞后获得了稳定表达克隆,且有目的蛋白表达,转染后细胞形态和生长特性发生了一定改变。结论获得了稳定表达NTN蛋白的Vero细胞株,为其移植并进行帕金森病猴基因治疗动物实验奠定了基础。
关键词(KeyWords)： Neurturin基因;Vero细胞;克隆;表达;帕金森病
基金项目(Foundation): 云南省科技强省计划基础研究重点项目(2007C0012Z)
作者(Authors): 马开利;孙茂盛;施锐;张莹;杨芳;奎翔;谭振国;李鸿钧;
DOI: 10.13200/j.cjb.2008.04.14.makl.023
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