人甲型H7N9禽流感全病毒灭活疫苗在小鼠中的免疫原性Immunogenicity of inactivated human avian influenza A H7N9 virus vaccine in mice
徐康维;于丹;邵铭;刘书珍;高强;李长贵;
摘要(Abstract)：
目的评价人甲型H7N9禽流感全病毒灭活疫苗在小鼠中的免疫原性。方法分别将H7N9与H1N1、H5N1全病毒灭活疫苗稀释至血凝素抗原(hemagglutinin,HA)浓度分别为30、6、1.2、0.24和0.048μg/ml后,免疫BALB/c小鼠,各10只,0.5 ml/只,采用血凝抑制(hemagglutinin inhibition,HI)试验检测免疫后小鼠血清抗体滴度,计算抗体几何平均滴度(GMT)及半数有效剂量(ED_(50)),并检测免疫后小鼠血清对其他亚型病毒的HI滴度。结果 H7N9组小鼠阳转率与H1N1组较相近,仅H7N9 0.6μg剂量组GMT明显低于H1N1同剂量组(P<0.001),其余剂量组差异无统计学意义(P>0.05);H7N9与H1N1、H5N1全病毒灭活疫苗免疫小鼠ED_(50)分别为0.27、0.12和15μg HA。免疫后血清对其他亚型病毒无交叉反应。结论 H7N9疫苗在小鼠中的免疫原性略低于H1N1疫苗,但明显优于H5N1疫苗。
关键词(KeyWords)： H7N9;流感疫苗;小鼠;免疫原性
基金项目(Foundation): 国家国际科技合作与交流专项(2013DFA31680)
作者(Author): 徐康维;于丹;邵铭;刘书珍;高强;李长贵;
Email:
DOI: 10.13200/j.cnki.cjb.001314
参考文献(References)：
- [1]GAO R,CAO B,HU Y,et al.Human infection with a novel avian-origin influenza A(H7N9)virus[J].N Engl J Med,2013,368(20):1888-1897.
- [2]WHO.Recommended composition of influenza virus vaccines for use in the 2015-2016 northern hemisphere influenza season[J].Wkly Epidemiol Rec.2015,90(11):97-108.
- [3]XU K W,YU D,SHAO M,et al.Study on immunogenicity of inactivated whole virion and split human avian influenza A(H7N9)vaccines in mice[J].Int J Biologicals,2015,38(3):105-107.(in Chinese)徐康维,于丹,邵铭,等.人甲型H7N9禽流感全病毒灭活疫苗和裂解疫苗在小鼠中的免疫原性研究[J].国际生物制品学杂志,2015,38(3):105-107.
- [4]XU K W,SHAO M,LIU S Z,et al.Rapid preparation of antiserum against influenza virus(H7N9)hemagglutinin for single radial immunodiffusion assay[J].Chin J Microbiol Immunol,2014,34(2):146-148.(in Chinese)徐康维,邵铭,刘书珍,等.人H7N9禽流感疫苗抗血清参考品的快速制备及初步应用[J].中华微生物学和免疫学杂志,2014,34(2):146-148.
- [5]LI C G,XU K W,HASHEM A,et al.Collaborative studies on the development of national reference standards for potency determination of H7N9 influenza vaccine[J].Hum Vaccin Immunother,2015,11(6):1351-1356.
- [6]LIU S Z,YAN K M,ZHOU L,et al.Immunogenicity of human avian influenza split vaccine to mice[J].Chin J Biologicals,2011,24(11):1310-1312.(in Chinese)刘书珍,闫昆明,周蕾,等.人禽流感裂解疫苗对小鼠的免疫原性观察[J].中国生物制品学杂志,2011,24(11):1310-1312.
- [7]TREANOR J J,CAMPBELL J D,et al.Safety and immunogenicity of an inactivated subvirion influenza A(H5N1)vaccine[J].N Engl J Med,2006,354(13):1343-1351.
- [8]LIN J T,ZHANG J S,DONG X P,et al.Safety and immunogenicity of an inactivated adjuvanted whole-virion influenza A(H5N1)vaccine:a phase I randomised controlled trial[J].Lancet,2006,368(9540):991-997.