乙型脑炎减毒活疫苗和灭活疫苗免疫效果评价Immune effects of live attenuated and inactivated Japanese encephalitis vaccines
刘欣玉;那瑞;俞永新;赵丹华;赵欣;徐宏山;李玉华;
摘要(Abstract)：
目的对目前市场上的乙型脑炎(简称乙脑)减毒活疫苗和灭活疫苗的免疫效果进行评价。方法将乙脑减毒活疫苗和灭活疫苗以相同剂量免疫小鼠14 d后,采集一部分小鼠血液,分离血清,检测中和抗体水平;另一部分用乙脑病毒强毒P3株进行腹腔攻击,观察小鼠存活情况,计算疫苗的保护率。结果乙脑减毒活疫苗和灭活疫苗原倍免疫小鼠后,抗体阳转率均为100%,乙脑病毒强毒攻击后保护率均为100%;乙脑减毒活疫苗1∶20稀释免疫小鼠后,抗体阳转率为90%,攻击保护率为100%。乙脑灭活疫苗1∶20稀释免疫小鼠后抗体阳转率为80%,乙脑病毒强毒攻击后保护率为70%。结论目前市场上应用的乙脑减毒活疫苗和乙脑灭活疫苗均是有效的,乙脑减毒活疫苗的保护效果更好。但通过接种不同的抗原量和采用不同的免疫程序,均可消除免疫保护效果的差异。
关键词(KeyWords)： 乙脑减毒活疫苗;乙脑灭活疫苗;免疫效果;攻击保护率
基金项目(Foundation):
作者(Authors): 刘欣玉;那瑞;俞永新;赵丹华;赵欣;徐宏山;李玉华;
DOI: 10.13200/j.cnki.cjb.002271
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