明胶微球乙型肝炎疫苗的研究Preparation of Gelatin Microsphere Encapsulated HB Vaccine
巩志立,赵晶,郑秋君,马靖,谢东生,张俊香,邓金娣
摘要(Abstract)：
用明胶作为原材料制备出10～100μm直径的明胶微球，用来包裹乙肝疫苗抗原，其包裹率为90％以上。免疫动物后抗－HBS在3～4个月达到高峰，10个月仍能维持较高水平（S／N为80～90）。微球疫苗优于Al佐剂疫苗，也优于含Al佐剂微球疫苗。明胶微球制备方法简单，抗原不接触有机溶剂。用琼脂糖、明胶＋阿拉伯胶等均能制成微球，且均可包裹成功。明胶微球可以成功地制备出HB－DT微球疫苗，关于它们的制备条件和免疫效果有待进一步观察和改进。
关键词(KeyWords)： 明胶微球;乙肝疫苗
基金项目(Foundation):
作者(Author): 巩志立,赵晶,郑秋君,马靖,谢东生,张俊香,邓金娣
Email:
DOI: 10.13200/j.cjb.1997.03.165.gongzhl.012
参考文献(References)：
- 1 Heeler J. Polymers for controlled perenteral delivery ofpeptide and protein. Advanced Drug Delivery Review,1993, 10: 163.
- 2 Saslawski O, Weingarten C, Benoit JP, et al. Magneli-cally responsive microspheres for the pulse delivery ofinsulin. Life Sci, 1988, 42: 1521.
- 3 康葵,等。当代结构药物全集,北京:北京科学技术出版社, 1993.
- 4 Eldrigde J. Biodegradable microspheres as a vaccinede-livery system. Mol Immunol , 1991, 28: 287.
- 5 Eldrigde J, Staas JK, Mealbroek JA, et al. Biodeg-radable and biocompatible poly (DL - Lactide - co -Glycolide ) microspheres as an adjuvant for staphylococ-cal enterotoxin B which enhances the level of toxin neu-tralizing antibodies. Infect Immun, 1991, 59: 2978.
- 6 Aguado M. Controlled releas vaccine: biogradable poly-lactide/polyglycolide PL/PG microspheres as antigenvehicles. Immunobio1ogy, 1992, 184: 113.
- 7 You C,Rill WL, Mal1l R, et al. Dependence of ricin tox-oid vaccine efficacy on the structure of poly (lactide-co-glycolide) micropatic1e carriers. Vaccine, 1995, 13(7): 645.
- 8 Nakaoka R, Tabata Y, IKada Y. et al. Potentiality ofgelatin microspheres as immunological adjuvant. Vac-cine, 1995, 13 (7): 653.