张丽娜;田九博;王萱;马培元;孟凡秀;文朝朝;魏艳;于保锋;

• 1:山西医科大学基础医学院

摘要(Abstract)：

目的分析赤子爱胜蚓Calreticulin的cDNA和氨基酸序列。方法从本院构建的赤子爱胜蚓cDNA文库中经测序获得其Calreticulin cDNA序列。采用生物信息学分析工具DNAMAN、NCBI ORF finder、ExPASy Protparam、ExPASY Protscale、NCBI Conserved Domains、SignalP 4. 1 Server、TMHMM Server v. 2. 0、SOSUI、NetPhos 3. 1 Server、PSORT、SOPMA、ExPASy COILS、SWISS-MODEL分别对Calreticulin的cDNA序列、氨基酸序列、一级结构基本理化性质、亲/疏水性、保守结构域、信号肽、跨膜区、可溶性、磷酸化修饰、亚细胞定位、二级结构、卷曲螺旋域以及三级结构进行预测及分析。结果赤子爱胜蚓Calreticulin cDNA序列长476 bp,其中包含354 bp的开放阅读框,编码118个氨基酸残基;Calreticulin由19种氨基酸组成,呈酸性,为稳定的可溶性亲水蛋白;具有1段Calreticulin超家族保守结构域;不存在信号肽和跨膜结构;具有12个潜在的磷酸化位点;主要存在于细胞核中;二级结构元件主要由α-螺旋(29. 66%)和无规则卷曲(50. 85%)组成,具有卷曲螺旋;三级结构预测结果与二级结构一致。结论掌握了赤子爱胜蚓Calreticulin的理化性质,预测了其潜在的特征,并分析了其结构,为后续深入开展其功能及分子作用机制的研究提供了理论依据。

关键词(KeyWords)： 赤子爱胜蚓;Calreticulin;cDNA;氨基酸;序列分析

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目（30472251）;; 山西省重点研发计划（201703D421023）

作者(Author): 张丽娜;田九博;王萱;马培元;孟凡秀;文朝朝;魏艳;于保锋;

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