王春燕;齐雪松;佟鹏;郝述霞;吕慧敏;苟巧;
WANG Chun-yan;QI Xue-song;TONG Peng;HAO Shu-xia;LV Hui-min;GOU Qiao;National Institute for Radiological Protection, Key Laboratory of Radiological Protection and Nuclear Emergency, Chinese Center for Disease Control and Prevention;

• 1:中国疾病预防控制中心辐射防护与核安全医学所辐射防护与核应急中国疾病预防控制中心重点实验室

摘要(Abstract)：

目的探讨白藜芦醇(resveratrol)衍生物白皮杉醇(piceatannol)和乙酰化白藜芦醇(acetylated resveratrol)对小鼠辐射损伤的防护作用。方法将昆明小鼠随机分为8组:正常对照组、照射对照组、白皮杉醇低、中、高剂量(50、100、200 mg/kg)组和乙酰化白藜芦醇低、中、高剂量(50、100、200 mg/kg)组,每组6只。小鼠经5.5 Gy 60Coγ射线一次性全身照射前2 h灌胃给药,对照组均灌服含2%DMSO的生理盐水溶液。照射后24 h,观察小鼠脾脏和胸腺系数、外周血淋巴细胞数量和百分率、血浆超氧化物歧化酶(superoxide dismutase,SOD)活力及骨髓嗜多染红细胞微核率的变化。结果与正常对照组相比,照射对照组小鼠脾脏和胸腺系数、外周血淋巴细胞数量和百分率及血浆SOD活力均显著降低(P<0.05或P<0.01),骨髓嗜多染红细胞微核率显著升高(P<0.01)。与照射对照组相比,白皮杉醇和乙酰化白藜芦醇各剂量组脾脏系数均显著增高(P<0.05或P<0.01),但胸腺系数差异无统计学意义(P>0.05);乙酰化白藜芦醇中、高剂量组外周血淋巴细胞数量和淋巴细胞百分率均显著升高(P<0.05或P<0.01);白皮杉醇中、高剂量组以及乙酰化白藜芦醇各剂量组血浆SOD活力均显著升高(P<0.05或P<0.01);白皮杉醇及乙酰化白藜芦醇各剂量组骨髓嗜多染红细胞微核率均显著降低(P<0.05或P<0.01)。结论白藜芦醇衍生物白皮杉醇和乙酰化白藜芦醇均对小鼠具有一定的辐射损伤防护作用,其作用机制与提高机体免疫能力、清除活性氧(reactive oxygen species,ROS)、保护造血组织免于辐射诱发的损伤有关。
Objective To investigate the protective effect of piceatannol and acetylated resveratrol on radiation damage in mice. Methods Kunming mice were randomly divided into eight groups, six for each. The mice in normal control group received no radiation, while those in the other seven groups received a single systemic radiation with 5. 5 Cy60 Co γ ray.The mice in normal control and model control groups were administered with physiological saline containing 2% dimethyl sulfoxide(DMSO) by lavage 2 h before radiation, while those in three piceatannol groups with piceatannol at dosages of50, 100 and 200 mg / kg, and those in three acetylated resveratrol groups with acetylated resveratrol at dosages of 50, 100 and 200 mg / kg, respectively. The coefficients of spleen and thymus, count and percentage of peripheral blood lympho-cytes, activity of plasma superoxide dismutase(SOD)and micronucleus(MN)rate of polychromatic erythrocytes in bone marrow were determined 24 h after irradiation. Results Compared with those in blank control group, the coefficients of spleen and thymus, count and percentage of peripheral blood lymphocytes and plasma SOD activity of mice in model con-trol group decreased significantly(P < 0. 05 or P < 0. 01), while the MN rate increased significantly(P < 0. 01). However,compared with those in model control group, the coefficients of spleen of mice treated with resveratrol or acetylated resver-atrol at various dosages increased significantly(P < 0. 05 or P < 0. 01), while those of thymus showed no significant difference(P > 0. 05). The counts and percentages of peripheral blood lymphocytes of mice treated with acetylated resvera-trol at moderate and high dosages increased significantly(P < 0. 05 or P < 0. 01). The plasma SOD activities of mice treated with piceatannol at moderate and high dosages and acetylated resveratrol at various dosages increased significantly(P < 0. 05 or P < 0. 01). The MN rates of mice treated with resveratrol or acetylated resveratrol at various dosages decreased significantly(P < 0. 05 or P < 0. 01). Conclusion Both piceatannol and acetylated resveratrol showed a certain protective effect on radiation damage in mice, of which the mechanism was associated with the improvement of immunity,clearance of reactive oxygen species(ROS)and protection of hemopoietic tissues against radiation-induced damage.

关键词(KeyWords)： 白皮杉醇;乙酰化白藜芦醇;辐射损伤;防护;小鼠
Piceatannol;Acetylated resveratrol;Radiation damage;Protection;Mouse

Abstract:

Keywords:

基金项目(Foundation):

作者(Authors): 王春燕;齐雪松;佟鹏;郝述霞;吕慧敏;苟巧;
WANG Chun-yan;QI Xue-song;TONG Peng;HAO Shu-xia;LV Hui-min;GOU Qiao;National Institute for Radiological Protection, Key Laboratory of Radiological Protection and Nuclear Emergency, Chinese Center for Disease Control and Prevention;

DOI: 10.13200/j.cnki.cjb.000849

参考文献(References)：

• [1]Ren JW,Yang Q.Protective mechanism of resveratrol on cerebral ischemia/reperfusion injury via ameliorating oxidative stress[J].Chin Pharmacol Bull,2011,27(4):448-451.(in Chinese)任俊伟,杨琴.白藜芦醇对脑缺血/再灌注氧化应激损伤保护机制的研究进展[J].中国药理学通报,2011,27(4):448-451.
• [2]EscotéX,Miranda M,Menoyo S,et al.Resveratrol induces antioxidant defence via transcription factor Yap1p[J].Yeast,2012,29(7):251-263.
• [3]Lu QJ,Wen LQ,Zhang M,et al.Study on radiation protective effect of resveratrol and its molecular mechanism[J].Chin J Radiol Med Prot,2004,24(1):21-22.(in Chinese)吕秋军,温利青,张敏,等.白藜芦醇的辐射防护及其分子机理的研究[J].中华放射医学与防护杂志,2004,24(1):21-22.
• [4]Herbert KJ,Snow ET.Modulation of arsenic-induced epidermal growth factor receptor pathway signalling by resveratrol[J].Chem Biol Interact,2012,198(1-3):38-48.
• [5]Mikula-Pietrasik J,Kuczmarska A,Rubis B,et al.Resveratrol delays replicative senescence of human mesothelial cells via mobilization of antioxidative and DNA repair mechanisms[J].Free Radic Biol Med,2012,52(11-12):2234-2245.
• [6]Li XX,Yan RA,Duan HY.Synthesis and antioxidant activity of piceatannol[J].Food Ferment Indust,2011,37(4):78-81.(in Chinese)李晓霞,晏日安,段翰英.白皮杉醇的合成及抗氧化活性研究[J].食品与发酵工业,2011,37(4):78-81.
• [7]Yang H,Baur JA,Chen A,et al.Design and synthesis of compounds that extend yeast replicative lifespan[J].Aging Cell,2007,6(1):35-43.
• [8]Frombaum M,Therond P,Djelidi R,et al.Piceatannol is more effective than resveratrol in restoring endothelial cell dimethylarginine dimethylaminohydrolase expression and activity after high-glucose oxidative stress[J].Free Radic Res,2011,45(3):293-302.
• [9]Wang CY,Hao SX,Tong P,et al.Radiation-protective effects of piceatannol and acetylated resveratrol[J].Carcinogen·Teratogen·Mutagen,2012,24(5):345-348.(in Chinese)王春燕,郝述霞,佟鹏,等.白藜芦醇衍生物白皮杉醇和乙酰化白藜芦醇辐射防护作用研究[J].癌变·畸变·突变,2012,24(5):345-348.
• [10]Jiang X,Lv XL,Li J,et al.Study on the radioprotection by purple sweet potato polysaccharide in vivo[J].J Food Sci Biotechnol,2010,29(5):665-669.(in Chinese)江雪,吕晓玲,李津,等.紫甘薯多糖对辐射的防护作用[J].食品与生物技术学报,2010,29(5):665-669.
• [11]Liu LH,Liu DX,Ma M,et al.Schisandra Chinensis extract on immune injury by radiation[J].Carcinogen·Teratogen·Mutagen,2012,24(2):108-110,115.(in Chinese)刘丽华,刘登湘,马鸣,等.五味子提取物预防辐射所致免疫损伤的实验研究[J].癌变·畸变·突变,2012,24(2):108-110,115.
• [12]Jiang YP,Wang SC,Zhao HP,et al.Effects of astragaloside on peripheral blood and bone marrow cells in mouse-treated with 60Co radiation[J].Pharm J Chin PLA,1999,15(3):6-11.(in Chinese)蒋永培,王胜春,赵慧萍,等.黄芪甲甙对60Co辐射小鼠外周血细胞和骨髓三系细胞的影响[J].解放军药学学报,1999,15(3):6-11.
• [13]Asadullina NR,Usacheva AM,Gudkov SV.Protection of mice against X-ray injuries by the post-irradiation administration of inosine-5'-monophosphate[J].J Radiat Res,2012,53(2):211-216.
• [14]Hosny Mansour H,Farouk Hafez H.Protective effect of Withania somnifera against radiation-induced hepatotoxicity in rats[J].Ecotoxicol Environ Saf,2012,80(1):14-19.