安宇;王颖;佐兆杭;易华西;张东杰;

• 1:黑龙江八一农垦大学
• 2:黑龙江省农产品加工与质量安全重点实验室
• 3:国家杂粮工程技术研究中心
• 4:中国海洋大学食品科学与工程学院

摘要(Abstract)：

目的从东北传统发酵肉制品中分离和再诱变选育广谱高产细菌素的植物乳酸菌,并对其进行鉴定。方法四轮复合诱变[微波诱变、亚硝基胍(nitroso-guanidin,NTG)诱变、常压室温等离子体(atmospheric room temperatureplasma,ARTP)诱变、紫外诱变突]具有降胆固醇能力的的植物乳杆菌M1株,获得还可产生广谱细菌素的植物乳杆菌。对其进行稳定性、微生物形态特征、生理生化特征及PCR鉴定。结果原始菌株经四轮复合诱变后获得1株高产细菌素菌株,该菌株兼具遗传稳定性、优良发酵特性和抑菌功效。鉴定该菌株为植物乳杆菌,命名为M1-UVs300,其分泌的细菌素命名为M1-UVs301;菌株保藏号为CGMCC No. 7972。结论通过四轮复合诱变获得的植物乳杆菌M1-UVs300抑菌谱较宽,且兼具降胆固醇和防腐的优良加工特性,工业化推广前景广泛。

关键词(KeyWords)： 植物乳杆菌;细菌素;复合诱变;分离;鉴定

Abstract:

Keywords:

基金项目(Foundation): 国家科技部重点研发计划(2017YFD0401203);; 国家“十二五”支撑计划-防腐保鲜新型物流包装材料开发(2015BAD-16B05);; 黑龙江八一农垦大学“三横三纵”科研团队支持计划-杂粮营养与质量安全创新团队(TDJH201806);; 黑龙江省优势特色学科资助项目(黑教联[2018]4号);; 黑龙江省农垦总局科技项目(HNK135-05-02)

作者(Authors): 安宇;王颖;佐兆杭;易华西;张东杰;

DOI: 10.13200/j.cnki.cjb.002513

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