2009年中国云南昆明地区柯萨奇病毒A组16型遗传特性分析Genetic characters of coxsackievirus A16 strain in 2009 in Kunming Region,Yunnan Province,China
马绍辉;陈俊英;李华;杨卉娟;施海晶;潘玥;孙强明;李琦涵;
摘要(Abstract)：
目的对2009年中国云南昆明地区柯萨奇病毒A组16型(Coxsackievirus A16,CoxA16)毒株的部分VP1区进行分析,了解CoxA16的遗传特性。方法设计针对肠道病毒A群的通用引物,采用半巢式RT-PCR扩增目的片段,对PCR产物测序;使用Omiga和Mega4.1等生物学软件进行核苷酸、氨基酸序列比对及病毒的系统进化分析。结果200份2009年昆明市儿童医院临床诊断为手足口病或并发脑炎疑似肠道病毒感染的患者粪便标本中有52份为CoxA16感染,其中15份来自脑炎患者。52株CoxA16病毒部分VP1区核苷酸和氨基酸序列同源性均较高,核苷酸序列同源性为98.5%～100%,氨基酸序列同源性为98.8%～100%;与shzh00-2、shzh99-48和107/Toyama/1990相比较,核苷酸序列同源性为92.3%～93.8%,氨基酸序列同源性为95.3%～96.8%;与国际标准株G10相比较,核苷酸序列同源性为78.5%～80.3%,氨基酸序列同源性为93.2%～94.5%;与其他13个参考株的核苷酸序列同源性为96.6%～99.5%,氨基酸序列同源性为98.3%～100%。基因系统进化分析显示,52株CoxA16均属于B基因型的一个分支。结论 2009年中国云南昆明地区CoxA16株属于B2亚型。
关键词(KeyWords)： 柯萨奇病毒A组16型;VP1;序列分析
基金项目(Foundation): 国家“艾滋病和病毒性肝炎等重大传染病防治-传染病新型疫苗研制(2008ZX10004-014)
作者(Author): 马绍辉;陈俊英;李华;杨卉娟;施海晶;潘玥;孙强明;李琦涵;
Email:
DOI: 10.13200/j.cjb.2012.01.14.mashh.001
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