林时辉;徐昉;刘琼;

• 1:重庆医科大学附属第一医院急诊科

摘要(Abstract)：

目的探讨姜黄素(Curcumin)对脓毒血症诱导的心肌功能障碍(Sepsis-induced myocardial dysfunction,SIMD)小鼠转化生长因子-β1(Transforming growth factor beta-1,TGF-β1)表达的影响。方法将SD小鼠随机分为假手术组(Sham组)、脓毒血症组(Sep组)、二甲基亚砜组(DMSO组)和姜黄素组(Cur组),Sep、DMSO和Cur组采用盲肠结扎穿刺术(Cecal ligation andpuncture,CLP)复制SIMD模型,造模24 h后,Cur组经腹腔注射200 mg/(kg.d)姜黄素,Sham和Sep组经腹腔注射等量生理盐水,DMSO组经腹腔注射等量DMSO。造模成功后0、6和12 h,各组小鼠经从右颈动脉插管使用BL-410多功能监护仪监测左心室收缩压(Left ventricular systolic pressure,LVSP)、左心室舒张末期压(Left ventricular end-diastolic pressure,LVEDP)、左室内压力变化最大上升和下降速率(Maximum rate of left ventricular pressure development and decay,LV±dp/dt-max);HE染色观察心肌组织病理变化;Western blot检测心肌组织中TGF-β1蛋白的表达。结果 Cur组小鼠24和72 h存活率均高于Sep组;心脏血流动力学指标(LVSP、LVEDP和LV±dp/dt-max)在各时间点与Sep组比较,均明显上升(P<0.05或P<0.01);各种心肌损害的病理学改变与Sep组相比,从6 h开始明显减轻;心肌组织中TGF-β1蛋白的表达量较Sep组明显下降(P<0.05)。各指标DMSO组与Sep组比较,差异均无统计学意义(P>0.05)。心肌病理学改变、心脏血流动力学变化及TGF-β1蛋白表达变化在各时相点存在一致性。结论姜黄素对SIMD小鼠的心肌保护作用与抑制TGF-β1蛋白的表达有关。

关键词(KeyWords)： 姜黄素;脓毒症;心肌功能障碍;转化生长因子β1

Abstract:

Keywords:

基金项目(Foundation): 重庆市卫生局医学科技计划项目(2009-2-332)

作者(Author): 林时辉;徐昉;刘琼;

Email:

DOI: 10.13200/j.cjb.2012.01.70.linshh.011

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