人参总皂苷对PC12细胞突起生长及GAP-43表达的影响Effect of total saponin of panax ginseng on neurite growth of PC12 cells and expression of growth-associated protein-43
李晗宇;王顺和;郑娟;姜蓉;
摘要(Abstract):
目的探讨人参总皂苷(Total saponin of panax ginseng,TSPG)对神经细胞PC12突起生长以及生长相关蛋白-43(Growth-associated protein-43,GAP-43)表达的影响。方法以0、10、20、40、80 mg/L TSPG作用于体外培养的PC12细胞,相差显微镜观察细胞的形态学改变,透射电镜观察细胞突触连接情况,显微镜测微尺测量突起长度,免疫组织化学和Western blot法检测GAP-43的表达。结果与对照组相比,TSPG处理组分化的PC12细胞直径明显增大,突起长度明显增长,并形成突触连接,GAP-43表达水平增加,以40 mg/L组最为明显。结论 TSPG可诱导PC12细胞发生分化、突起生长延伸,并可上调GAP-43的表达水平。
关键词(KeyWords): 人参总皂苷;神经元;突起生长;GAP-43蛋白
基金项目(Foundation):
作者(Author): 李晗宇;王顺和;郑娟;姜蓉;
Email:
DOI: 10.13200/j.cjb.2012.01.52.lihy.009
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