柳蕾;徐兴丽;冯敏;何玉凤;王丽春;张莹;李琦涵;

• 1:中国医学科学院北京协和医学院医学生物学研究所云南省重大传染病疫苗研发重点实验室
• 2:昆明医科大学

摘要(Abstract)：

目的比较单纯疱疹病毒Ⅰ型(herpes simplex virus typeⅠ,HSV-Ⅰ)基因突变株M3株与水痘带状疱疹病毒(varicella-zoster virus,VZV)减毒活疫苗Oka株感染BALB/c小鼠的生物学特性。方法用不同剂量(103、104、105 PFU/只)的HSV-ⅠM3株及疫苗剂量的VZV Oka株以不同方式(腹腔、肌肉、鼻腔、足垫)感染小鼠后,对小鼠的急性临床反应、体重变化、病毒增殖、组织病理损伤及病毒重激活等生物学特性进行比较。结果 HSV-ⅠM3及VZV Oka组小鼠均未见弓背、倒毛、单侧眼失明等急性临床症状,体重均呈逐渐上升趋势;器官组织中病毒拷贝数均处于较低水平,且两组差异无统计学意义(P> 0. 05);小鼠脑组织出现轻微局部炎症细胞浸润、出血、胶质小结或无异常,两组差异较小,脊髓组织均未出现明显异常;神经组织与Vero细胞共培养,均未观察到细胞病变(CPE)。结论 HSV-ⅠM3株与VZV Oka株感染小鼠后的生物学特征无明显差异。

关键词(KeyWords)： 单纯疱疹病毒Ⅰ型;突变株;水痘带状疱疹病毒;减毒活疫苗;小鼠;生物学特性

Abstract:

Keywords:

基金项目(Foundation): 中国医学科学院医学与健康科技创新工程项目(2016-I2M-1-019);; 国家自然科学基金(31670173);; 云南省科学技术厅(2017ZF006);; 云南省重大专项(2017ZF020);; 北京协和医学院“协和青年基金”(3332016115);北京协和医学院研究生创新基金项目(2017-1001-05)

作者(Authors): 柳蕾;徐兴丽;冯敏;何玉凤;王丽春;张莹;李琦涵;

DOI: 10.13200/j.cnki.cjb.002510

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