血脂康对新生大鼠心肌成纤维细胞增殖及胶原合成的影响Effect of Blood Fat Regulator Xuezhikang on Proliferation and Collagen Synthesis of Cardiac Fibroblast of Newborn Rats
赵淑健;金玉怀;叶平;邵宁生;杨光;刘坤申;
摘要(Abstract)：
目的观察血脂康对血管紧张素Ⅱ(AngⅡ)诱导的新生大鼠心肌成纤维细胞(CFs)增殖及胶原合成的影响,并探讨可能的分子机制。方法采用胰酶消化法分离培养Sprague-Dawley大鼠心肌成纤维细胞,建立AngⅡ诱导CFs增殖的模型,以MTT比色法和流式细胞仪检测细胞周期分析法观察血脂康对CFs数目和细胞周期的影响。AngⅡ及不同浓度血脂康作用48 h后,用天狼星红染色法检测培养上清中胶原的含量;ELISA法检测细胞培养上清液中TGF-β1蛋白表达;RT-PCR法检测胶原和TGF-β1 mRNA表达。结果AngⅡ对CFs增殖有明显促进作用,血脂康可明显抑制AngⅡ诱导的CFs增殖,且呈剂量依赖性;血脂康可增加G0/G1期细胞百分率,降低S、G2/M期细胞百分率,降低胶原含量、TGF-β1蛋白及胶原和TGF-β1 mRNA的表达。结论血脂康能抑制AngⅡ诱导的CFs增殖和胶原的产生,其作用可能是通过抑制TGF-β1表达实现的。
关键词(KeyWords)： 血脂调节剂;心肌成纤维细胞;增殖;胶原;血管紧张素Ⅱ
基金项目(Foundation):
作者(Authors): 赵淑健;金玉怀;叶平;邵宁生;杨光;刘坤申;
DOI: 10.13200/j.cjb.2008.04.67.zhaoshj.006
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